Insegnamento
LABORATORY OF ADVANCED DNA, RNA AND PROTEIN ANALYSIS
AVP5073637, A.A. 2016/17

Principali informazioni sull'insegnamento
Corso di studio Corso di laurea magistrale in
BIOTECNOLOGIE PER L'ALIMENTAZIONE
IF0362, ordinamento 2011/12, A.A. 2016/17
1140178
Curriculum BIOTECHNOLOGIES FOR FOOD SCIENCE [002LE]
Crediti formativi 9.0
Denominazione inglese LABORATORY OF ADVANCED DNA, RNA AND PROTEIN ANALYSIS
Sito della struttura didattica http://www.agrariamedicinaveterinaria.unipd.it
Dipartimento di riferimento Dipartimento di Biomedicina Comparata e Alimentazione (BCA)
Sito E-Learning https://elearning.unipd.it/scuolaamv/course/view.php?idnumber=2016-IF0362-002LE-2016-AVP5073637-N0
Obbligo di frequenza No
Lingua di erogazione INGLESE
Sede LEGNARO (PD)

Docenti
Responsabile BARBARA CARDAZZO VET/04
Altri docenti ALESSANDRO BERTOLI BIO/10

Dettaglio crediti formativi
Tipologia Ambito Disciplinare Settore Scientifico-Disciplinare Crediti
CARATTERIZZANTE Discipline biotecnologiche comuni BIO/10 4.0
CARATTERIZZANTE Discipline veterinarie e riproduzione animale VET/04 5.0

Modalità di erogazione
Periodo di erogazione Secondo semestre
Anno di corso I Anno
Modalità di erogazione frontale

Organizzazione della didattica
Tipo ore Crediti Ore di
Corso
Ore Studio
Individuale
Turni
ESERCITAZIONE 3.0 24 51.0 Nessun turno
LABORATORIO 3.0 24 51.0 Nessun turno
LEZIONE 3.0 24 51.0 Nessun turno

Calendario
Inizio attività didattiche 27/02/2017
Fine attività didattiche 09/06/2017

Commissioni d'esame
Commissione Dal Al Membri
1 Commissione A.A. 2016/17 01/12/2016 30/11/2017 CARDAZZO BARBARA (Presidente)
BERTOLI ALESSANDRO (Membro Effettivo)
FASOLATO LUCA (Supplente)
NOVELLI ENRICO (Supplente)

Syllabus
Prerequisiti: Theoretical knowledge: principles of Biochemistry (academic level), with particular focus on protein Biochemistry. Basis of molecular genetics and molecular biology methods.
Practical skills: basic skills for a laboratory of Biochemistry and Molecular Biology (e.g., knowledge of safety rules, use of micro-pipettes, preparation of solutions).
Conoscenze e abilita' da acquisire: Students will learn the theoretical and practical principles of methodologies for the identification of novel biological markers through proteomics approaches (in particular, 2D-electrophoresis) and theoretical and practical principles of molecular biology methods.
Modalita' di esame: Written dissertation on the theoretical and practical contents of the course, with particular emphasis on protein 2D-electrophoresis and related techniques for the identification of biological markers. In some instance, a PowerPoint presentation of a scientific paper will also be envisaged.
Criteri di valutazione: Students shall demonstrate to have acquired the principles and skills previously described, and to be able to apply them in the identification of biomarkers (in particular, in the field of food control).
Contenuti: 1. Proteomics
Recollections of protein biochemistry. Protein structure and structure-function relationship, forces that drive protein folding and protein-protein interactions, differences between hydro-soluble and membrane proteins.
Definition and goals of proteomics. Properties of the proteome; identification of biological markers by proteomic analyses; advantages and disadvantages of the proteomic approach in comparison with other techniques. Overview on the most common methodologies in proteomics.
Theoretical and practical principles of two-dimension gel electrophoresis (2D-electrophoresis). General principles of protein electrophoresis; isoelectric point and isoelectrofocusing; SDS-PAGE.
Techniques for protein extraction, solubilization and purification. Extraction of proteins from the biologic milieu; protein denaturation; preparation of the sample for the 2D-electrophoresis; reduction and alkylation.
Protein identification. Gel staining; tryptic digestion; liquid phase chromatography and mass spectrometry (hints); peptide fingerprinting and sequencing; database mining. Immuno-based techniques for protein identification.
Other techniques in proteomics. The ETTAN-DIGE® technique for 2D-electrophoresis. Techniques for membrane protein identification. Protein microarrays and antibody microarrays. Recent mass spectrometry techniques in proteomics (shotgun proteomics).
Lab sessions: By exploiting principles and techniques learned in the first part of the course, each student will accomplish a complete experiment of 2D-electrophoresis, from protein extraction to gel staining.

2. Molecular biology
Introduction to Molecular Biology methods and application to food analysis.

Description of Methods: Extraction of nucleic acids, PCR and real-time PCR: theoretical principles and application, sequencing methods (Sanger, Next generation sequencing, RNA seq).

Molecular Biology lab session: Molecular typing of bacterial isolates, application of real-time PCR. Next-Generation Sequencing technologies.
Attivita' di apprendimento previste e metodologie di insegnamento: Classes (8 hours) will serve to introduce the principles and techniques described in the previous Section, part of which will be used in the laboratory sessions.
Proteins
During laboratory classes (24 hours) students will be split into small groups (3-4 people), each of which, during a one-week intensive program (4-5 hours a day), will carry out a complete experiment of 2D-electrophoresis, under the teacher’s supervision.
Molecular biology
During laboratory classes (32 hours) students will be split into small groups (3-4 people), each of which, will carried out the different laboratory experience described in Course contents section under the teacher’s supervision
Eventuali indicazioni sui materiali di studio: The material used for the course will be made available to students through the Moodle platform of the School at:
https://elearning.unipd.it/scuolaamv/login/index.php


Additional notes and scientific papers will be provided by the teacher
Testi di riferimento: