2004-present Researcher in Cell Biology, Department of Biology, University of Padova
1999-2003: Research fellow, Department of Biology, University of Padova
1998-1997: Two EMBO short-term Fellowship, Department of Biochemistry, Karolinska Institute, Stockholm, Sweden
1996-1998: PhD in Evolutionary Biology, University of Padova
1995: Erasmus Fellowship, Department of Genetics, University of Stockholm, Sweden
1994: Laurea in Biological Sciences, University of Padova
1969: Born in Padova
CR has a specific expertise of mammalian cells in culture and molecular cell biology dating back to her time as a student when she detected by DNA fingerprinting the induction of mutations by recombinogenic agents in an established cell line.
She first became interested in the regulation of DNA precursors (dNTPs) in mammalian cells when she was an Erasmus fellow at Stockholm University. She first studied the enzymes involved in dNTP de novo synthesis and salvage during the cell cycle and then turned to the study of 5'-nucleotidases. The function of these enzymes in cell metabolism was then poorly defined, although there were indications that nucleotidases participate in the regulation of dNTP pools. She cloned for the first time the whole coding sequence of human high-Km 5'-nucleotidase (cNII) and expressed it in bacteria and mammalian cells. She studied the in vivo properties of the enzyme and demonstrated its participation in purine ribonucleotide regulation. She worked for extended periods in the laboratory of Prof. Reichard in Stockholm where she purified from human placenta the cytosolic deoxyribonucleotidase (cdN) and cloned its cDNA. By constructing overproducing cell lines she demonstrated that cdN participates in a substrate cycle regulating pyrimidine dNTPs. In 1999 on the basis of sequence homology with cdN she discovered the first known deoxynucleotidase in mitochondria (mdN). She cloned and expressed the protein and studied its enzymic properties. The tridimensional structure of mdN was solved in collaboration with the group of Prof. P. Nordlund, Stockholm University. The first 3D structure of a eukaryotic 5'-nucleotidase, it allowed to outline a detailed reaction mechanism that can be applied to all other members of the 5'-nucleotidase family. In engineerd cell lines that overproduce mdN or have its activity knocked-down by RNA interference she has shown that mdN operates in an intramitochondrial substrate cycle regulating mitochondrial dTTP synthesis. In collaboration with Prof. J. Balzarini, Catholic University of Leuven, she demonstrated that the susceptibility of a nucleotide analog to be dephosphorylated by cdN and mdN is a predictive factor of the drug efficiency in anticancer or antiviral therapy.
Following the discovery of mdN and the reports of mitochondrial diseases due to disregulation of DNA precursors, she became interested in the mechanisms regulating mitochondrial DNA maintenance. She studies the mechanisms that in mammalian cells keep the dNTP pools in balance, focusing on the enzymes that contribute to pool regulation by degrading dNTP precursors.
Insegnamenti dell'AA 2016/17
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